The Center bases its work on the following instrument: 96-capillary 3730xl DNA Analyzer
GeneMapper® Software can be purchased through the local agent Rhenium (www.rhenium.co.il)
Peak Scanner™ 2 Software can be free downloaded from here:
The two dye sets for detection and sizing labeled PCR product are available in the Center:
DS-30: Size standard ROX-400. PCR product should be <400bp and labeled with 6-FAMTM, HEXTM, NEDTM.
DS-33: Size standard LIZ-500. PCR product should be <500bp and labeled with 6-FAMTM, VICTM, NEDTM, PETTM.
All samples should be submitted with Sample submission form for labeled PCR products
1. PCR is performed by customer
Labeled PCR amplification products are submitted.
PCR products should arrive in a 96-wells plate even if the plate is not full.
The order of the samples in the plate should always be as follows:
Sample 1 is in position A1
Sample 2 is in position B1
Sample 96 is in position H12
Samples should be diluted appropriately before submission.
Table with names of the samples should be send by E-mail.
2. PCR is performed at the Center
- DNA samples are submitted. Concentration of DNA >100ng/µl. The samples should arrive in 1.5ml tubes.
- Primers should be provided by the customer. The concentration of the primers should exceed 10µM and be noted on the tube.