|C1™ Single-Cell Auto Prep System
The Fluidigm C1 Single-Cell Auto Prep System based on microfluidic technology and is used to capture and prepare individual cells for genomic applications.
Steps of cells preparation:
- Single cells capture into 96 individual chambers
- Direct cell lysis
- Reverse transcription of RNA
- cDNA preamplification
The multiplex preamplification products are harvested, and transferred to the BioMark™ HD System
for quantitative PCR analysis or to library preparation for next generation sequencing.
Types of C1 System-compatible IFCs (Integrated Fluidic Circuits):
For Preamplification (PreAmp) protocol
for small (5-10 μm), medium (10-17 μm, and large (17-25 μm) single cells,
For mRNA Seq protocol
for medium (10-17 μm) and large (17-25 μm) single cells.
Single-Cell Gene Expression
Single-Cell Targeted Gene Expression
Single-Cell miRNA Profiling
Single-Cell mRNA Sequencing Analysis