The Alexander Silberman Institute of Life Sciences
The Center for Genomic Technologies : Bioanalyzer

The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells.
The Agilent 2100 bioanalyzer is the industry standard for RNA sample QC and has replaced gel electrophoresis for this application. It is also rapidly replacing gel electrophoresis for DNA fragment analysis and SDS-PAGE analysis of protein samples
Sample submission
The samples should arrive only after telephone appointment with the Center. The samples should be in RNase free 1.5ml tubes.

RNA quality check
RNA analysis for total RNA, mRNA and Small RNA samples by consuming only 1 µL of sample even pg amounts.
The integrity of total RNA samples is estimated by RIN algorithm (RNA Integrity Number)

DNA size and quantity
Smart, high resolution separation and quantification of DNA

The RNA integrity number (RIN) is a software tool designed to help scientists estimate the integrity of total RNA samples.
RNA degradation is a gradual process. As degradation proceeds there is a decrease in the 18S to 28S ribosomal band ratio and an increase in the baseline signal between the two ribosomal peaks and the lower marker.
The RIN software algorithm allows for the classification of total RNA based on a numbering system from 1 to 10, with 1 being the most degraded profile and 10 being the most intact.

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