Expression analysis and RNA profiling

The Center bases its work on the following instruments:

Affymetrix Software in the Center:

Affymetrix® GeneChip® Command Console® Software (AGCC)  summarizes probe cell intensity data (.CEL file generation).

Available applications:

Complete list of applications and arrays (https://www.thermofisher.com/il/en/home/life-science/microarray-analysis.html)

 

For details please contact the local Affymetrix Product manager:

Amos Grundwag, Eisenberg Bros. Ltd.,

Tel: 972-3-9777037, Mobile: 972-52-8910960,

E-Mail: amosg@eisenbros.co.il

 

Sample submission:

 All samples should be submitted with Sample Submission Form for Affymetrix Microarrays.

 

RNA/DNA requirements:

RNA

For Eukaryotic Expression assay: 50-500ng (concentration > 50ng/μl)

For Prokaryotic Expression assay: 10-13μg (concentration >500ng/μl)

For Whole Transcript Sense Target assay: 300ng (concentration > 100ng/μl)

For miRNA assay: 0.2-3μg (concentration > 125ng/μl)

 

Ratio 260/280=1.8-2.0 

RIN (RNA Integrity Number)>7 is acceptable

  

DNA

For CytoScan assay: >300ng (concentration > 50ng/μl)