The Alexander Silberman Institute of Life Sciences
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Bio-Imaging Unit

Academic head: Prof. Michael Brandeis

Facility director: Dr. Naomi Melamed-Book

Tel:  02-6585453
Fax: 02-6585844


The Transmission Electron Microscopy Unit (TEM)

Head: Dr. Yael Friedmann

Team: Hava Glickstein, M.Sc

Tel:  02-6586031         
Fax: 02-6585844

The Electron Microscopy unit provides services, equipment, expertise and support for researchers in the Life, Material and Medical Sciences.

The services include:
-    Preparation of the biological samples for viewing: Fixing, sectioning, staining,   viewing and documenting the results.
-    Immuno-electron microscopy using pre-embedding and post-embedding methods
-    Negative staining.
-    Preparation and sectioning of non-biological materials for researchers at the Chemistry and Physics Institutes.

The unit is equipped with:
•    TEM Tecnai 12 equipped with a CCD camera MegaView II and software AnalySIS 3.0.
•    LKB III ultra microtomes, two units
•    Ultracut S Reichert ultra microtome
•    Carbon & shadowing Edwards 306 coater
•    Glow discharge unit


Advanced Light Microscopy

Head:
Dr. Naomi Melamed-Book
Tel:   02-6585453
Fax:  02-6585844

The Bio-Imaging Unit at the Institute for Life Sciences is equipped with the following light microscopes: 

1.    Olympus FV1000 confocal - Inverted Microscope with live cell imaging capabilities.
2.    Leica SP5 confocal - Upright Microscope.
3.    Olympus FV1200 confocal - Inverted Microscope with live cell imaging capabilities.
4.    Fully motorized wide field fluorescent microscope.
5.    Hermes WiScan high throughput system.
6.    Computers and programs for image analysis.
 

FV-1000 Confocal Microscope
 The FV-1000 confocal microscope is equipped with 4 lasers, 4 fluorescent filter based detectors and a transmitted light detector. The system includes two scan heads so that imaging and bleaching can be done simultaneously.
The available lasers are: 405nm, multiline argon ion laser (457nm, 488nm, 514nm, 561nm and 633nm.
Our FV-1000 confocal is suitable for live cell imaging. It is equipped with an on-scope incubator (Life Imaging Services) which controls temperature, CO2 concentration, and humidity. The system is also equipped with an auto-focus (ZDC) system.

Leica SP5 Confocal Microscope
The Leica SP5 confocal microscope is a upright confocal microscope and its equipped with 5 lasers, 4 fluorescent spectral detectors and a transmitted light detector.
The available lasers are: 405nm, multiline argon ion laser (457nm, 488nm, 514nm, 561nm, 594nm and 633nm.

FV-1200 Confocal Microscope
The FV-1200 confocal microscope is equipped with 5 lasers, 4 fluorescent filter based detectors and a transmitted light detector. The system includes two scan heads so that imaging and bleaching can be done simultaneously.
The available lasers are: 405nm, 488nm, 514nm, 561nm and 633nm.
Our FV-1200 confocal is suitable for live cell imaging. It is equipped with an on-scope incubator (Life Imaging Services) which controls temperature, CO2 concentration, and humidity. The system is also equipped with an auto-focus (ZDC) system.

Motorized Wide-Filed Fluorescent Microscope
Our wide field fluorescent microscope is based on an IX81 inverted scope (Olympus), it is equipped with a x-cite light source and emission filters (regular and fast) for DAPI, CY2, CY3, Texas rex and Cy5 and transmitted light.

Hermes WiScan - High Throughput Microscope System
The system is a fast imaging system equipped with 7 color scanning capabilities, automatic focusing, temperature and CO2 controls.
The system scans multi well plates as well as slides.

Image Analysis
Computers for Image analysis are available as well as programs for analyzing the data. The programs are: Fiji (Imagej), Imaris, Volosity.


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Faculty of Science The Hebrew University of Jerusalem The Hebrew University of Jerusalem