The Alexander Silberman Institute of Life Sciences
Home
The Center for Genomic Technologies : MiSeq sequencing

MiSeq Benchtop Sequencer
 
MiSeq is the benchtop sequencer that can produce 2 x 300 paired-end reads and up to 15 Gb of data in a single run.
 
Sequencing applications: 
  • Whole-genome DNA sequencing
  • De novo sequencing
  • Targeted resequencing
  • Amplicons
  • Whole Transcriptome RNA sequencing
  • PolyA mRNA sequencing
  • Small RNA analysis
  • Methylation analysis
  • Protein-nucleic acid interaction analysis (ChIP-Seq)

 


 

Specifications
MISEQ REAGENT KIT V2
READ LENGTH
TOTAL TIME*
OUTPUT
1 × 36 bp
~4 hrs
540-610 Mb
2 × 25 bp
~5.5 hrs
750-850 Mb
2 × 150 bp
~24 hrs
4.5-5.1 Gb
2 × 250 bp
~39 hrs
7.5-8.5 Gb
MISEQ REAGENT KIT V3
READ LENGTH
TOTAL TIME*
OUTPUT
2 × 75 bp
~24 hrs
3.3-3.8 Gb
2 × 300 bp
~65 hrs
13.2-15 Gb
 
MISEQ REAGENT KIT V2
Single Reads
12-15 M
Paired-End Reads
24-30 M
MISEQ REAGENT KIT V3
Single Reads
22-25 M
Paired-End Reads
44-50 M
 
Advanced Search


 
 
Faculty of Science The Hebrew University of Jerusalem The Hebrew University of Jerusalem