The Alexander Silberman Institute of Life Sciences
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Confocal Microscope Unit

Scientific Director: Prof. Joseph Orly
Facilty Director:  Dr. Naomi Melamed-Book
Contact info:
Phone:  02-6585453
Fax:       02-6586448


The confocal unit in the Institute for Life Sciences has two confocal microscopes in use:


Confocal Microscope image

 
FV-1000 Confocal Microscope
 
The FV-1000 confocal microscope is equipped with 4 lasers, 4 confocal detection channels, and one transmitted light detector. The system includes two laser scanners so that bleaching and imaging can be done simultaneously. .
The available lasers are: 1) blue diode laser (405nm), multiline argon ion laser (457nm, 488nm, 514nm), green and red Hene lasers (543nm and 633nm).
 
The second scanner ("SIM" scanner) is well suited for FRAP experiments, where the 405nm laser can bleach a small, defined area, while any of the other lasers can be used through the main scanner to simultaneously image the sample. This configuration of the microscope is also useful for uncaging experiments, using the 405nm laser.
 
Our FV-1000 confocal is suitable for live cell imaging. it is equipped with an on-scope incubator (Life Imaging Services) which controls temperature, CO2 concentration, and humidity.
 
Other capabilities of the system include: ability to do XYZT acquisition with multiple fluorescence channels and over multiple fields (multipoint). The zero drift compensation (ZDC) system allows the system to maintain focus over long timelapse experiments. This will often eliminate the need to acquire multiple focal planes, thus reducing exposure and the resulting phototoxicity. 
 
 
 
MRC-1024 Confocal Microscope
 
The BioRad confocal has 2 lasers: an Argon Ion Laser (457, 488and 514nm wave lengths) and a 594nm HeNe laser .
It has 3 confocal detectors (PMT) and one transmitted light detector. Any combination of three detectors can be selected at one time.
People who have been trained to operate the MRC-1024 (usually after a few training sessions) can use it on their own.
To see conditions and terms of use please go click here.
The Confocal unit also provides assistance in designing experiments, including selection of dyes and combinations of dyes, choice of imaging modes and data acquisition, and image analysis and visualization. Researchers are strongly encouraged to discuss their experiments with the confocal unit staff in advance.
 
Software currently available and supported by the confocal unit includes Image Pro Plus (Media Cybernetics) this program can only be used in the unit and Image J which can be installed in any computer. Our ImageJ implementation includes the most commonly needed functions, including the ability to read the image data provided by the confocal unit.
We also suggest using the FV Viewer for the FV1000 files.
 
To download a version of Image J (with all necessary plugins) click here Image J
 

 
Fees:
  • 25$/ hour (during the day, both confocals for members of the Life Science institute and faculty members).
  • 35$/ hour for other academic users.
  • 70$/ hour for companies and industry.
  • 5$/ hour live cell imaging (over night for members of the Life Science institute and faculty members).
  • 10$/hour live cell imaging for other academic users.
 
 
 
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Faculty of Science The Hebrew University of Jerusalem The Hebrew University of Jerusalem